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ENZYMES & Chemical Digestion

ENZYMES & Chemical Digestion. Chemical Digestion Basics. Dehydration Synthesis vs. Hydrolysis Anabolic – Dehydration Synthesis Require the removal of water to form bonds Catabolic – Hydrolytic Reactions Require the addition of water to break bonds Carbohydrates – mono and disaccharides

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ENZYMES & Chemical Digestion

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  1. ENZYMES&Chemical Digestion

  2. Chemical Digestion Basics • Dehydration Synthesis vs. Hydrolysis • Anabolic – Dehydration Synthesis • Require the removal of water to form bonds • Catabolic – Hydrolytic Reactions • Require the addition of water to break bonds • Carbohydrates – mono and disaccharides • Lipids (triglycerides) – glycerol + fatty acids • Proteins – amino acids

  3. Terminology: • Enzyme • Active site • Substrate(s) • Product(s) • Reversible reactions substrate enzyme products enzyme

  4. Factoids: Enzymes are: • Proteins

  5. B + A Factoids: Molecule C Enzymes: • Lower the activation energy needed for a reaction to take place

  6. Factoids: Enzymes: • Are unchanged after catalyzing a reaction

  7. Factoids: Enzymes: • Turnover numbers are very high • Examples: • 1 molecule of acetylcholinesterase -> 25,000 molecules of acetylcholine in 1 second • 1 molecule of catalase breaks down 40 million molecules of hydrogen peroxide in 1 second

  8. Factoids: Enzymes: • Are specific in their activity • Lock and key model

  9. Enzymes: Sometimes require a nonprotein factor = cofactor (metallic ion) or coenzyme (vitamin) Examples: Iron, zinc, copper, sodium, potassium, vitamin B Factoids:

  10. Enzymes: are usually named after the substrate on which they act have names ending in –ase ( with a few exceptions) Examples: Cellulase Sucrase Lipase Lactase Acetylcholinesterase Factoids:

  11. Factors Affecting Enzyme Activity

  12. Temperature

  13. pH more H+ Pepsin – stomach enzyme Trypsin – duodenum enzyme

  14. Concentrationof Enzyme or Substrate

  15. Inhibitors • Competitive Inhibition • Noncompetitive Inhibition

  16. Enzyme Uses

  17. Papaya and Papain

  18. Pineapple and Bromelain

  19. Lactose = milk sugar • Lactase – enzyme produced by cells that line the small intestine • Symptoms • Solutions • Avoid dairy products (calcium problem) and prepared foods containing lactose • Determine problematic amounts of lactose or source (i.e. milk, ice cream, aged cheeses) • Lactase additives: add to milk (lactose content reduced by 70% or buy lactose-reduced milk

  20. Enzyme Dietary Supplements

  21. Questions Concerning Dietary Enzyme Supplements • Are there people who do not produce enough of certain enzymes? • Could an increase in digestive enzymes increase the digestion of food? • Can you increase the amount of digestive enzymes by taking oral dietary supplements?

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