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Chiba iGEM 2007

Chiba iGEM 2007. Marimo. What the hell is Marimo?. Marimo (Japanese: 毬藻 marimo ), also known as Cladophora ball, Lake ball, or Moss Balls in English, is a species of filamentous green algae ( Chlorophyta ) found in a number of lakes in the northern hemisphere. blabla…. １. ２. ３.

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Chiba iGEM 2007

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  1. Chiba iGEM 2007 Marimo

  2. What the hell is Marimo? • Marimo (Japanese: 毬藻marimo), also known as Cladophora ball,Lake ball, or Moss Balls in English, is a species of filamentous green algae (Chlorophyta) found in a number of lakes in the northern hemisphere. • blabla…

  3. １ ２ ３ まりも完成 To make a bacterial Marimo

  4. Our System • Gather bacteria • Quorum Sensing • Stick bacteria together • flagella: fliC gene • Control size (advanced) • Tune Quorum Sensing

  5. Ｒ Ｒ ＡＨＬ Ｒ Ｒ Ｒ Ｒ Ｒ Ｒ Ｒ Ｒ Ｓ ＡＨＬ Ｒ Ｓ Ｓ Ｒ ＡＨＬ Ｒ Ｓ Ｒ Ｒ Ｒ Ｒ Ｒ ＡＨＬ Ｒ Ｒ Ｒ Ｒ Ｒ Detailed Design

  6. Detailed Design: Sender Cell • Constitutively expressflagella • Produce AHL

  7. Detailed Design: Reciever Cell • LuxP > flagella • no AHL > aiiA ??

  8. Ｒ Ｒ ＡＨＬ Ｒ Ｒ Ｒ Ｒ Ｒ Ｒ Ｒ Ｒ Ｓ ＡＨＬ Ｒ Ｓ Ｓ Ｒ ＡＨＬ Ｒ Ｓ Ｒ Ｒ Ｒ Ｒ Ｒ ＡＨＬ Ｒ Ｒ Ｒ Ｒ Ｒ Detailed Design: Overview ①Ｓｅｎｄｅｒは常に鞭毛が生えていて、ＡＨＬを生産する。 ②ＲｅｃｅｉｖｅｒはＡＨＬ存在下で鞭毛が生えて、ＡｉｉＡによりＡＨＬを分解する。 ③鞭毛にはヒスタグが付いているのでニッケルイオンを仲介としてくっつく。

  9. Experiments & Results Quorum sensing, FliC-his gene

  10. Experiments • Quorum Sensing • modify? for a lab-class 自由溶液 • size-tunable • Flagella (fliC) engeneering

  11. Quorum Sensing in a liquid… • To make the AHL conc. gradation • Sender(=AHL producer) Problem • Senderを固める（拡散させない） • 必要量のAHLを出すSenderの数 • AHL拡散 Problem • solvent’s viscosity • AHL溜る -> 分解する(aiiA)

  12. Size Control • Super Sender Cell • Produce more AHL • Super Reciever Cell • Sensitive

  13. To Make a Marimo • Check the flagella adsorption w/Quorum sensing • 2-dimensional test @ plate • 3-dimensional (marimo) • Size control test

  14. Controling Marimo Size

  15. What we learned

  16. Acknowledgements

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