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  1. FIGURE 18.1 Types of Contamination. Examples of microorganisms found to contaminate cell cultures.(a) Bacteria. (b) Yeast. (c) Mold. (d) Mycoplasma colonies growing on special nutrient agar. (e, f ). Scanning electron micrograph of mycoplasma growing on the surface of cultured cells. (See Plate 16e, f for mycoplasma stained with Hoechst 33258) d–f , courtesy of Dr. M. Gabridge; a–c, 10x objective.)

  2. FIGURE 18.2 Mycoplasma Detection by PCR. Ethidium bromide fluorescence of PCR products of infected, uninfected, and control cells, electrophoresed on 1.3% agarose. The 100-bp ladder consists of the following bands: 100, 200, 300, 400, 500 (strongly stained band), 600, 700, 800, 900, 1031, 1200, 1500, 2000, and 3000 bp. The wild-type mycoplasma bands are about 510 bp, and the internal control band is almost 1000 bp. (Courtesy of Cord Uphoff, DSMZ)

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