Institute of Microbiology Chinese Academy of Sciences

1. Institute of Microbiology Chinese Academy of Sciences • Microbial Resources • Applied & Industrial Microbiology • Pathogenic Microbiology

2. Advisory Committee Academic Committee International Advisory Panel Academic Degree Committee National Advisory Panel Development Committee Center for Microbial Resources State Key Lab of Microbial Resources CAS Key Lab of Syst. Mycol. & Lichenol. Center for Agri. Biotech. Co-op with Inst. Genetics & Develop. Biol., CAS Center for Molecular Immunology Center for Environ. Biotech. Center for Molecular Virology Center Microb. Metab. Eng. Center for Microbial Genome Research Co-op with Beijing Genomics Inst., CAS Center for Energy & Industrial Biotechnology Culture Collect. Center High-throughput Screening Platform Tech. Support Platform Microbial Analy. Center Information Center National Microbial Engineering Center Administrative System

3. Developmental Strategy • Construct Modern Biotechnology Platforms • Human Resources • Co-op with Industry • International Co-op

4. Broad International Co-operation

5. MultiGenome Access Technology for Industrial Catalysts (MGATech) An EU - China Collaboration Review of FP5 in IMCAS European Commission Fifth Framework RTD Programme Quality of Life and Management of Living Resources Key Action 3: The Cell Factory RTD Priority 3.3.4: Identification and Sustainable Use of Metabolic and Genetic Diversity as a Source of New Valuable Products

6. MultiGenome Access Technology for Industrial Catalysts An EU – China Collaboration European Commission Fifth Framework RTD Programme Thematic Programme : Quality of Life and Management of Living Resources Key Action 3 : The Cell Factory RTD Priority 3.3.4 : Identification and Sustainable Use of Metabolic and Genetic Diversity as a Source of New Valuable Products Project Number: QLK3-CT-2002-01972 Hydrothermal outbreak, upper site small pool Rehai thermal area Overview Novel genetic methods will be developed for accessing gene products derived from Chinese hot springs and salt lakes to which we have unique access in a collaboration with the Chinese authorities. These Chinese extreme environments are of outstanding significance as microbial ecosystems with unique chemistries. The sites harbour largely unexplored microbial genetic resources that will be harnessed as sources of new and valuable enzymes. Gene libraries reflecting the microbial biodiversity at these exceptional sites will be employed for the identification and production of valuable new enzymes for sustainable applications in biotechnological processes and consumer products. This special collaboration, which is the first microbial biotechnology collaboration between the EU and China, will provide substantial opportunities for the development of European and Chinese biotechnology. Pearl Spring – Rehai thermal area Objectives • Obtain material from a range of unique Chinese hypersaline and thermal sites • Survey microbial biodiversity, and establish and archive a collection of novel microbes • Develop methods for the efficient isolation and recovery of microbial genes from these sites • Identify and produce high added value new biomolecules derived from these sites and assess these new biomolecules for commercial exploitation Sampling algal mat at large basin Langpu geothermal area

7. 极 端 微 生 物 实 验 室 Deliverables and Achievements • Sample Acquisition: The mounting of expeditions and collection of samples/nucleic acids from seldom accessed thermal and hypersaline sites. – The first expedition to the Yunnan Province (Rehai area) was completed in March 2003 and the second expedition to Inner Mongolia will take place in September 2003. • Gene Acquisition: We will recover microbial genes from a variety of samples, some partially processed on site . There has been good progress with the isolation of DNA from geothermal sites visited during the first sampling expedition and we have begun to generate bacterial gene libraries derived from these sites. The first gene library derived from “Higher Microbes” (i.e. fungi, algae and protozoans) has been constructed from a hot spring algal mat. • Organism Acquisition: We will generate and archive culture collections of microbes from these sites. The first joint paper by Consortium members has been submitted for publishing approval. • Enzyme Acquisition: We will detect and assess industrially-relevant enzymes from the genetic resources derived from these expeditions and technologies. Hydrothermal outbreak pool Rehai thermal area The Consortium • Prof. Bill Grant • University of Leicester, UK • Prof. Antonio Ventosa • University of Sevilla, Spain • Prof. Don Cowan • University of the Western Cape, South Africa • Prof. Ma Yanhe • Institute of Microbiology, Chinese Academy of Sciences • Prof. Brian Jones • Genencor International BV, The Netherlands The Sampling Team Contacts Coordinator: Prof. Bill Grant, EU Scientific Officer: Dr. Indridi Benediktsson University of Leicester, UK Indridi.benediktsson@cec.eu.int wdg1@le.ac.uk

8. Extremozyme genes • DNA library • Metagenomic DNA Libraries • cDNA Library High thoughput screening -Mannanase -Amylase -Xylanase Lipase Catalase …….

9. Design of an Integrated Environmental Clean Industrial Fungal Process Integrating Water Management for the Valorisation of Agricultural Wastes in Paper Industry An EU - China Collaboration Review of FP4 in IMCAS European Commission Fourth Framework RTD Programme Thematic Programme:Basic Natural Resources Project number:ERBIC18CT980284

10. Design of an Integrated Environmental Clean Industrial Fungal Process Integrating Water Management for the Valorisation of Agricultural Wastes in Paper Industry Objectives: 1.Water pollution prevention: Development of a fungal enzyme process using mainly laccases for improving annual plant kraft pulp bleachability. 2.Water pollution treatment and recycling: Design of a fungal process for effluent detoxification and decolourization in order to facilitate the subsequent biological treatment and water recycling. European Commission Fourth Framework RTD Programme Thematic Programme : Basic Natural Resources Project Number: ERBIC18CT980284 Coordinator: Prof.Dr. Jean-Claude Sigoillot Institut National De La recherche Agronomique jcs@esil.univ-mrs.fr The Consortium: -DR.GUIGAN FANG CHINESE ACADEMY OF FORESTRY -DR.HEINZ JELLER ANDRITZ AG, AUSTRIA -DR.LIN GUO INSTITUTE OF MICROBIOLOGY, CAS Activities: To achieve these objectives, the proposed project has been constructed with an integrated multidisciplinary approach combining complementary Chinese and European industrial partners including small enterprises in close association with academics originating from both countries.

11. Ongoing International Cooperative Projects • Design new chromegens Oxoid-Fisher Limited,UK • Biodiversity and conservation of important bio-resources Royal Botanic Gardens, Kew, UK • Biodiversity of mycorrhyzal bacteria in forests of China and UK Oxford University,UK • Acidophilic actinomycetes as a source of new natural products University of Newcastle, UK

12. IMCAS look forward to establishing the long-term cooperationwith EU An EU – China Collaboration

13. Thank You