1 / 14

An example of Flow Cytometric DNA Analysis as a diagnostic tool

An example of Flow Cytometric DNA Analysis as a diagnostic tool. J. Chezar Western Galilee Hospital-Nahariay Israel. History:

alta
Download Presentation

An example of Flow Cytometric DNA Analysis as a diagnostic tool

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. An example of Flow Cytometric DNA Analysis as a diagnostic tool J. Chezar Western Galilee Hospital-Nahariay Israel

  2. History: A 49 year old male referred to the hematology department because of pancytopenia. After 2 dry taps, a bone marrow aspirate resulting in barely 200 uL of material was obtained and sent for flow cytometry. The sample was stained with the following antibody combinations. CD45PC7/CD34ECD/CD33 PC5/ CD117 PE/ HLA-DR FITC, CD45PC7/CD3ECD/CD56PC5/CD8PE/CD4FITC+CD19FITC, CD45PC5/CD13PE/CD16FITC, CD45PC5/CD64PE/CD14FITC. DNA cell cycle analysis was performed alone and in combination with CD45 FITC and glycophorin A FITC, CD13 FITC. In addition a bone marrow biopsy was sent to pathology. Immuno-histochemistry was done with peroxidase conjugated LCA, MPO, CD34, TdT, CD15, CD117, PAS, CD43, CD138, CD31, CD79a, Factor VIII, Ker, CD68, CD3, B and T cell antibodies

  3. BLASTS

  4. Aberrant population Normal Bone Marrow Patient Bone Marrow

  5. Myeloid phenotype = Blasts?

  6. MPO: GRAN+ / BLASTS -

  7. PAS NEG IN BLASTS

  8. Normal 2n Aneuploid (A)

  9. To verify identity of aberrant population – double stain with PI for DNA and relevant FITC labeled antibody

  10. A Aneuploid population NOT leukocytic

  11. A Aneuploid population NOT thrombocytic

  12. A Glycophorin A + erythroid !! Aneuploid population IS

  13. Glycophorin

  14. Erythro Leukemia DNA analysis and subsequent double staining with appropriate antibodies lead to the correct diagnosis.

More Related