1 / 26

Phytochemical profiling of different genotypes of strawberries

Phytochemical profiling of different genotypes of strawberries. Sara Tulipani, Marche Polytechnic University Ancona, Italy. Material: List of cultivars. STORAGE Short refrigeration (3 days at 4°C + 1 day at RT). PhD Project. NUTRITIONAL QUALITY of STRAWBERRY fruits:

hye
Download Presentation

Phytochemical profiling of different genotypes of strawberries

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Phytochemical profiling of different genotypes of strawberries Sara Tulipani, Marche Polytechnic University Ancona, Italy

  2. Material: List of cultivars STORAGE Short refrigeration (3 days at 4°C + 1 day at RT)

  3. PhD Project NUTRITIONAL QUALITY of STRAWBERRY fruits: From the characteristics of the FRUIT to the effects on HUMAN BODY • Phytochemical and antioxidant profiling of the fruits • Studies on Bioavailability • Studies on Bioefficacy

  4. PhD Project • Phytochemical and antioxidant profiling of the fruits STSM • Studies on Bioavailability • Studies on Bioefficacy

  5. Before the STSM........ • Already available informations: • Total antioxidant capacity and vitamin C contribution • Data about total anthocyanins, total phenolics, total folate content • Preliminary studies on human volunteers

  6. STSM at PRI Institute • Moisture Content (Oven method) • Vit C content (HPLC) • Phytochemical & Antioxidant profile (HPLC coupled to online antioxidant analysis) • Additional analysis • Technical explorations

  7. 1. Moisture level (Oven method) • Range from 87.4% to 94.2%

  8. 2. Vitamin C content (HPLC) Genotype-to-genotype differences

  9. 2. Vitamin C content (HPLC) Effect of storage

  10. 3. HPLC coupled to on-line antioxidant analysis Koleva I I et al. Anal Chemistry 2001, 73, 3373-3381

  11. 3. HPLC coupled to on-line antioxidant analysis Koleva I I et al. Anal Chemistry 2001, 73, 3373-3381

  12. 3. Phenolic composition by HPLC-PDA • 254 nm: ellagic acid derivatives • 280 nm: phenolic acids • 312 nm: phenolic acids (p-coumaric acid derivatives) • 360 nm: flavonols • 512 nm: anthocyanins

  13. An example of antioxidant analysis……

  14. An example of antioxidant analysis……

  15. HPLC On-line antioxidant analysis

  16. Genotype-to-genotype differences

  17. HPLC On-line antioxidant analysis

  18. HPLC On-line antioxidant analysis

  19. Genotype-to-genotype differences RT = 13.8 p-coumaric acid glucoside RT = 14.7 p-coumaric acid glycoside M D B

  20. Genotype-to-genotype differences

  21. HPLC On-line antioxidant analysis

  22. Genotype-to-genotype differences Pg-glucoside Cy-glucoside A Pg-rutinoside Pg-malonyl glucoside G B

  23. HPLC On-line antioxidant analysis Pg-glucoside: 25 to 40% of the total antioxidant contribution (excluding vitamin C)

  24. Phytochemical composition and antioxidant contributions Effect of storage • No common trends on the variation of compounds in all clones • (No relevant effects, increase, decrease of single classes of compounds)

  25. Conclusions 9 strawberry genotypes: • Dry matter content variation • 2-fold differences on the vitamin C content • Variability on the phenolic composition on a qualitative and quantitative basis • Anthocyanins: the main contribution to TAC (vitamin C excluded) • Others polyphenols: variation on the relative antioxidant contribution

More Related