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12-3 DNA Replication

12-3 DNA Replication. Federoff. Eukaryotic DNA Replication. Step 1 – Helicase unwinds and unzips the DNA molecule. Step 2 – DNA Polymerase adds on complementary nucleotides in a 5’ to 3’ direction.

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12-3 DNA Replication

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  1. 12-3 DNA Replication Federoff

  2. Eukaryotic DNA Replication • Step 1 – Helicase unwinds and unzips the DNA molecule.

  3. Step 2 – DNA Polymerase adds on complementary nucleotides in a 5’ to 3’ direction.

  4. Step 3 – The lagging strand continues to replicate in fragments instead of continually like the leading strand.

  5. Okazaki Fragments

  6. Step 4 – Since the fragments still aren’t joined, the enzyme ligase, joins the fragments.

  7. Step 5 – As replication continues, the leading and lagging strand twist back into their helical form.

  8. Telomeres • Are the tips of chromosomes that make it less likely important genes will be lost with replication.

  9. Prokaryotic DNA Replication • Starts at a single point, and proceeds in 2 directions until the entire chromosome is copied.

  10. Prokaryotic vs. Eukaryotic • DNA Replication • DNAMovie • Prokaryotic DNA Replication

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