1 / 18

Digital Microscopy : Improving Identification & Quantification in a Freshman Biology Classroom

Digital Microscopy : Improving Identification & Quantification in a Freshman Biology Classroom. Elizabeth Smith West Aurora High School RET fellow, 2010 University of Illinois-Chicago NSF Grant CBET-EEC-0743068. Outline. My RET experience My School, My Students Technology:

nonnie
Download Presentation

Digital Microscopy : Improving Identification & Quantification in a Freshman Biology Classroom

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Digital Microscopy:Improving Identification & Quantification in a Freshman Biology Classroom Elizabeth Smith West Aurora High School RET fellow, 2010 University of Illinois-Chicago NSF Grant CBET-EEC-0743068

  2. Outline • My RET experience • My School, My Students • Technology: • Present & Future • Objectives & Standards • Lessons & Applications (6) • Grant Request • Acknowledgements • References & Resources

  3. Background: My RET at UIC • Dr. Michael Cho’s lab • Effects of Extracellular Topography on Human Mesenchymal Stem Cells

  4. Background: My School, My Students

  5. Background: My School, My Students • ALL students take some Biology • Biology: • 50/50 Freshman & Sophomores • Most students: • Read below grade level • ~30% of students have parents that attend Open House or Conferences or attempt to contact the teacher

  6. Technology Current Technology Current Challenges • 9 Biology classrooms • Each has computer + LCD projector • 6 Compound Light Microscope sets • 13 scopes each • 2 scope cams • Technology: • Cameras are old, only show B/W images • TVs are small, whole class can’t see • Students: • Less than half have used a microscope • Students have little concept of what they are seeing!

  7. Technology A Solution MOTOCAM 1000 • USB-based microscope ($700+) OR • USB-based microscope camera ($350+) 1.3 Mega Pixel camera • Calibration slide for accurate measurements Software: Motic Images 2.0 • Capture stills & video • Measurements (including area) • JPG, etc. to email/print • Combine images • Image processing • & more!

  8. Objectives • Use the MOTOCAM 1000 to: • Show the whole class proper microscope technique • Capture videos for later use & posting on school website • Includes narration software • Capture images for statistical analysis • Quantifying Biology! Compatible with Excel

  9. Illinois Standards

  10. Teaching: Microscopy Techniques Intro to Microscopes Digital Microscope Application • Students learn how to use a microscope • Wet mount slides • Letter “e” • Crossed threads • Printed paper • Misc. prepared Slides (bugs, etc) Analyze • Which way is the image facing? • What happens when you move the slide left? • Why can’t you focus on both threads at once? • Show students what “in focus” looks like! • Avoid the “Bubble Eureka!” moment

  11. Teaching: Plant v. Animal Cells Comparing Cell Structures Digital Microscope Application • Have students create & examine 3 cell samples: • Onion skin cell • Elodea cell • Human Cheek cell Analyze • Compare & Contrast the 2 plant cells • Compare & Contrast the plant v. animal cell • The first time that many students see cells! • Most have no idea what the are seeing: which things are cells?! • Capture stills • Great for referring back or for absent students • Capture video of moving chloroplasts

  12. Teaching: Osmosis Watching Water Move Digital Microscope Application • Using Elodea samples, create a slide, observe • Flood the slide with saline solution, observe Analyze • What happens to the cell? • How could you reverse the process? • Record video samples of the lab to • share with absent students & • when teaching about hyper-/hypo-tonic solutions

  13. Teaching: Mitosis & the Cell Cycle Seeing Mitosis Digital Microscope Application • Use Onion Root tip or other premade mitosis slides • Students find & sketch examples of interphase, mitosis (PMAT), and cytokinesis • Identify cell membrane, cell wall, chromosomes, cleavage furrow, cell plate Analyze • Compare & contrast mitosis in plant & animal cells • Before lab, use digital projector to identify the parts of mitosis in real cells • In lab, have students take turns capturing & printing images of different parts of the root tip. • Count the # in each phase • Create a graph to show number of cells in each phase • Analyze: Which phases are the longest? Shortest?

  14. Teaching: Pond Water & Protists Protists in the Water! Digital Microscope Application • Depression slide + pond water, • Sketch, identify, and label the structures. • Cell membrane, cytoplasm, chloroplasts, cilia, flagella, nucleus Analyze • Beside each, describe: • Unicellular or multicellular? • Autotrophor heterotroph? • How does this protist move? • How does this protist feed? • Have the class work as a group to capture an image of each new organism • Volvox & ameobae are rare most years; capture a video to share with other classes • Use pictures captured to label in later assessments

  15. Teaching: Plant Adaptations Anatomy of a Leaf Digital Microscope Application • Have students exam cross sections of a dicot leaf; draw & label: • Cuticle, epidermis, pallisidemesophyll, spongy mesophyll, vascular bundle (xylem + phloem) stomata, guard cells Analyze • Describe how each helps with photosynthesis or protection • Have each group capture a picture of the epidermis from a tropical & a desert biome • Count the number of stoma in the same size area • What differences do you see? Why?

  16. Technology Requested • Quote by Scope Shoppe (scopeshoppe.com) 750 Every Year Total students affected?

  17. Acknowledgements • NSF Grant CBET-EEC-0743068 • Prof. A. Linninger, RET Program Director • Dr. Michael Cho, Research Mentor • Brandon Lutz and Hannah Wirtshafter, fellow researchers • University of Illinois- Chicago

  18. References & Resources • Illinois Interactive Report Card • http://iirc.niu.edu/ • Swift Optical Resources • http://www.swiftoptical.com/EducationalResources.aspx • Scope Shoppe • www.scopeshoppe.com • Pictures • http://www.subbody.net/01subbody/Unfold/240/071013_volvox.jpg • http://www.kuhnphoto.com/gallery/biology/microscopic/stomata.jpg • http://faculty.irsc.edu/FACULTY/TFischer/bio%201%20files/test-yourself%20mitosis.jpg • https://kleinsclasses.wikispaces.com/file/view/handbook-microscope_noshadow-T.jpg/56048204/handbook-microscope_noshadow-T.jpg • http://www.workshopplus.com/productcart/pc/catalog/moticam1000setup_809_detail.jpg • http://www.tedpella.com/cameras_html/camera2.htm • http://www.ndt-educational.org/images/Artefatti26.jpg • http://wikidoc.org/images/a/a6/Chloroplasten.jpg

More Related