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Distribution of house dust mites in Yangsan, Changnyeong, Sancheong and Geochang Area

Distribution of house dust mites in Yangsan, Changnyeong, Sancheong and Geochang Area Hui-Geun Cha , Jong-Hwa Chung, Yeong-Hun Kang, Gang-Ja Ha, Yeong-Hoon Kim and Jung-Ung Park Gyengsangnam-do Health & Environmental Research Institute. Abstracts.

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Distribution of house dust mites in Yangsan, Changnyeong, Sancheong and Geochang Area

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  1. Distribution of house dust mites in Yangsan, • Changnyeong, Sancheongand GeochangArea • Hui-GeunCha, Jong-HwaChung, Yeong-Hun Kang, • Gang-JaHa, Yeong-Hoon Kimand Jung-UngPark • Gyengsangnam-do Health & Environmental Research Institute

  2. Abstracts During the period of 2000.1-2000.12, house dusts were collected from 140 houses with electric vacuum cleaners. House dust mites were isolated from 2.5g dust by applying the modified wet sieving method. Total 67,011 mites were collected and 4 suborder 8 family 9 genus 10 species were identified. Among them, Dermatophagoidesfarinae(DF) was 45,568 mites(68.00% of the total) and predominant, followed byD. pteronyssinus(DP) 18,776 mites(28.02%) and Tyrophagusputrescentiae(TP) 2,185 mites(3.26%). DFwas predominant in Sancheong(53.8%), Changnyeong(64.9%) and Geochang(86.7%), whereas DPwas predominant in Yangsan(50.5%). Among 140 study houses, DF, DPand TP were found in 41.4%, co-habitat of two species in 45.0% and one species in 11.4%. DFwas predominant in 75.7% of total samples, DPin 19.3% and TP in 3.6%. In 2.5g of the house dust, less than 99 mites were found in 48 houses(35.0%), 100-499 mites in 51 houses(36.4%), 500-999 mites in 20 houses (14.3%) and more than 1,000mites in 17 houses(12.2%). In order to evaluate environmental factors affecting the population density of house dust mites, house type, age of house construction, size of the house and number of the family were compared with the number of mites, and none of the above factors statistically affected with the mite density.

  3. Purpose of Experiments □ Distribution of house dust mites by areas, house type and number of family □ Evaluation of environmental factors with mite density □ Isolation and identification of house dust mite Material and Method □ Period : 2000. 1. ∼ 2000. 12. □ Sample : Total 140 vacuum cleaner filter bags □ Area : Yangsan, Changnyeong, Sancheongand Geochang □ Habitat : Apartments and private house □ Method : Modified wet sieving method

  4. Collection vacuum cleaner Filter bags ↓ Dust 2.5g ↓ Add 50% EtOH 500-600ml ↓ Shake 5min 12mesh(top part), 200mesh(bottom part) sieve ↓ Washing Collect 200mesh sieve dust ↓ Add 80% EtOH 500-600ml ↓ Washing 3-5min Saturated saline water 500ml ↓ 30min Supernant solution layers ↓ Centrifuge 1,300-1,500rpm, 5min ↓ Filter paper Observation by microscope ↓ Identification dust mites ↓ Manufacture mite sample Fig 1. Procedure for identification of house dust mites.

  5. R e s u l t s Table. Result of mite collections from house dusts at 4 locations in Gyengnam

  6. Table. Average number(percent) of three major species of house dust mites at 4 locations in Gyengnam Number/2.5g dust/house a)DF : Dermatophagoides farinae b)DP : Dermatophagoides pteronyssinus c)TP : Tyrophagus Putrescentiae

  7. Table. Average number(percent) of three major species of house dust mites at 4 locations in Gyengnam Number/2.5g dust/house a)DF : Dermatophagoides farinae b)DP : Dermatophagoides pteronyssinus c)TP : Tyrophagus Putrescentiae

  8. Table. Co-habbitat of three predominant species(DF, DP and TP) at 4 locations in Gyengnam

  9. Table. Co-habbitat of three predominant species(DF, DP and TP) at 4 locations in Gyengnam

  10. Table. Number(percent) of houses in which DF, DP and TP was predominant species

  11. Table. Number of houses with mite densities (average number of mites per 2.5g of the coarse house dust)

  12. Table. Comparison of house dust mite densities between apartments and private house Table. Comparison of house dust mite densities among different sizes of the house

  13. Table. Comparison of house dust mite densities among different family sizes Table. Comparison of house dust mite densities among different ages of house construction

  14. Conclusion • . • Total 67,011 mites were collected and 4 suborder 8 family 9 genus 10 species • were identified. • 2. Dermatophagoidesfarinae(DF) was 45,568 mites(68.00% of the total) and predominant, followed byD. pteronyssinus(DP) 18,776 mites(28.02%) and • Tyrophagusputrescentiae(TP) 2,185 mites(3.26%). • 3. DFwas predominant in Sancheong(53.8%), Changnyeong(64.9%) and Geochang(86.7%), whereas DPwas predominant in Yangsan(50.5%). • 4. Among 140 study houses, DF, DPand TP were found in 58 houses(41.4%), co-habitat of two species in 63 houses(45.0%) and one species in 16 houses(11.4%). • 5. DFwas predominant in 75.7% of total samples, DPin 19.3% and TP in 3.6%. • 6. In 2.5g of the house dust, less than 99 mites were found in 48 houses(35.0%), 100-499 mites in 51 houses(36.4%), 500-999 mites in 20 houses(14.3%) and • more than 1,000mites in 17 houses(12.2%). • Environmental factors(the population density of house dust mites, house type, age of house construction, size of the house and number of the family) were • statistically not affected with the mite density.

  15. References 1. Oshima S : Studies on the mite fauna of the house dust of Japan and Taiwan with special reference to house dust allergy. Jap. J. Sanit. Zool., 21 : 1-17, 1970. 2. Wharton GW : Mites and commercial extracts of house dust. Science 167 : 1382-1383, 1970 3. Voorhorst R, Spieksma-Boezeman MIA and Spieksma FThM; Is a mite(Dermatophagoides sp.) the producer of the house dust allergen? Allerg. Asthma, 10: 329-333, 1964. 4. Maunsell, K, Wraith, D.G, and Cunnigton, A.M. : Mites and house dust allergy in bronchial asthma. Lancet 1 : 1267-1270, 1968. 5. Pepys. J., Chan, M. and Hargreave, F.E. : Mites and house dust allergy. Lancet 1 : 1270-1272. 1968. 6. Buchanan, D.J and Jones, I.G : Allergy to house dust mites in the tropics Brit. Med J. 3 : 764, 1972. 7. Warner, J.O., Price, J.F., Soothill, J.F. and Hey, E.N. : Controlled trial of hyposensitization to Dermatophagoides pteronyssinus in children with asthma. Lancet 1 : 912-915. 1978.

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