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How were the yeast cells extracted?

Learn about four extraction methods for yeast cells, including detailed steps and chemical compositions of aqueous and lipid extracts. Explore the differences between the extracted compounds and their potential applications.

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How were the yeast cells extracted?

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  1. How were the yeast cells extracted? • Method 1: • Quench: -80°C, 80% MeOH • Extract: 80% MeOH, 100% Chloroform 30 minutes at room temperature • Method 2: • Quench: -80°C, 80% MeOH • Extract:-80°C,80% MeOH, 100% Chloroform 30 minutes in a dry ice/EtOH bath • Method 3: • Quench: ice-cold dH2O • Extract: -80°C, 80% MeOH, 100% Chloroform 30 minutes in a dry ice/EtOH bath • Method 4: • Quench: ice-cold PBS • Extract: -80°C, 80% MeOH, 100% Chloroform 30 minutes in a dry ice/EtOH bath

  2. AQUEOUS EXTRACT Black: Extraction A; Red: Extraction B; Blue: Extraction C; Purple: Extraction D

  3. AQUEOUS EXTRACT M1 (Group A Aqueous) methanol trehalose threonine valine glutamine glutarate glycine propylene glycol succinate alanine citrate glutamate lactate carnitine arginine isoleucine acetone leucine lycine acetate serine glutathione UDP-glucose asaparagine isobutyrate tyrosine proline aspartate phenylalanine fumarate NAD+ formate

  4. AQUEOUS EXTRACT formate phenylalanine tyrosine tyrosine UDP-glucose NAD+ NAD+ NAD+ UDP-glucose NAD+ fumarate NAD+ NAD+ NAD+ UDP-glucose

  5. AQUEOUS EXTRACT Black: Extraction A; Red: Extraction B; Blue: Extraction C; Purple: Extraction D

  6. LIPID EXTRACT L1 (Group A Aqueous)

  7. LIPID EXTRACT Black: Extraction A; Red: Extraction B; Blue: Extraction C; Purple: Extraction D

  8. LIPID EXTRACT Black: Extraction A; Red: Extraction B; Blue: Extraction C; Purple: Extraction D

  9. LIPID EXTRACT Black: Extraction A; Red: Extraction B; Blue: Extraction C; Purple: Extraction D

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