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Extraction of Nicotine

Extraction of Nicotine . By: DJ Hall and Brittany Hicks. Purpose. Determine if nicotine is extractable by means of supercritical fluid Run more than one sample type to determine if this makes a significant difference. SFE. Supercritical Fluid Extraction Uses high pressure CO 2

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Extraction of Nicotine

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  1. Extraction of Nicotine By: DJ Hall and Brittany Hicks

  2. Purpose • Determine if nicotine is extractable by means of supercritical fluid • Run more than one sample type to determine if this makes a significant difference

  3. SFE • Supercritical Fluid Extraction • Uses high pressure CO2 • Used to extract organics from different types of matrixes • Allows for easy separation • Problems • All soluble organics will be separated form matrix

  4. GC-MS • Gas Chromatography Mass Spectroscopy • Separation of violate organics • Determine the analyte separated at specific retention times using MS

  5. Nicotine (162.12g/mol) • Poison (parasympathomimetic alkaloid) • Formed in nightshade family of plants • Antiherbivor chemical • Small doses • Stimulant • Relaxation • Large doses • Fatal

  6. Extraction • 2 Cigarettes Marlboro blend 27 • 2 Pieces Nicorette Gum 2mg • 50 Drops of e-cigarette liquid 2.4% by weight

  7. Extraction cont. • All extractions were obtained by bubbling into methanol and using SFE methanol modifier pump • Cigarettes • Ground using mortar and pestle • Nicorette Gum • Cut into small pieces • E-cigarette liquid • Celite as inert matrix

  8. Determination of Nicotine • All samples were diluted to 50ml of methanol • A standard was created by diluting 4mg of Nicotine in 50ml of methanol • Standards and Samples were run using the GC-MS

  9. Reference Mass Spectra

  10. Standard Nicotine peak

  11. Cigarettes Nicotine peak

  12. Cigarettes cont.

  13. E-Cigarette Liquid Nicotine peak

  14. E-Cigarette Liquid Cont.

  15. Nicorette Gum

  16. Chromatogram Comparison E-Cigarette Liquid Nicorette Gum Cigarette Standard

  17. Conclusion • It is possible to extract nicotine using the SFE • Both the E-Cigarette and Cigarette samples contained nicotine • The Nicorette Gum showed undetectable amounts of nicotine • Nicotine may have be contained in the gum residue that was filtered before dilution

  18. Future Project • Quantify the amounts of nicotine extracted • Determine the extraction efficiency

  19. Work Cited • Fischer, M. and Jefferies, T. Optimization of Nicotine Extraction from Tobacco Using Supercritical Fluid Technology with Dynamic Extraction Modeling. J.Agric. Food Chem. (1996), 44, 1258-1264.

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