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Biological Membranes

Biological Membranes. Penghui Lin Nov. 15. 2013. Membranes. http://www.uvm.edu/~inquiryb/webquest/fa06/mvogenbe/. Diffusion across plasma membrane. http://en.wikipedia.org/wiki/Cell_membrane. Plasma Membrane.

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Biological Membranes

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  1. Biological Membranes Penghui Lin Nov. 15. 2013

  2. Membranes http://www.uvm.edu/~inquiryb/webquest/fa06/mvogenbe/

  3. Diffusion across plasma membrane http://en.wikipedia.org/wiki/Cell_membrane

  4. Plasma Membrane Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  5. Man-made membrane http://bioweb.wku.edu/courses/biol115/wyatt/biochem/lipid/Lipid_2.asp

  6. Liquid crystals • Nematic phase • Smecticphases • Chiral phases http://en.wikipedia.org/wiki/Liquid_crystal

  7. Sample preparation Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)

  8. Specular Neutron Reflectivity Incident angle = reflected angle Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)

  9. Density profile ρ0(z) Fourier Synthesis Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)

  10. Lipid bilayer phases

  11. Location of Cholesterol Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  12. Location of cholesterol Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  13. Forces between membranes Osmotic pressure Disjoining pressure Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  14. Osmotic stress experiment Neutron/ Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  15. Thermal unbinding Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  16. Single component membrane Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  17. Lipid mixtures and domains • Ideal mixing: no phase separation • Non-ideal or non-random mixing: compositional fluctuation happens while no stable domains formed • Macroscopic phase separation: micron-size stable lipid domains are formed

  18. No phase separation Membrane thickness Fluctuation parameter in DMPC bilayers at 35° Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  19. Macroscopic phase separation DPPC/DOPC/cholesterol Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  20. Supramolecular properties Tilted phase Interdigitated phase Diffused scattering Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  21. Bicelle mixtures Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  22. Bicelle mixtures Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  23. Fluctuation change upon antimicrobial peptide binding Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)

  24. Membrane rigidity change Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  25. Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  26. Thanks! Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)

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